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In a side project, we tested whether PCR probes designed based on wheat EST sequences can be used to identify BAC clones carrying those genes. Barrels Build Kits Gunsmith Work Lower Parts Lower Receivers Rifles Upper Parts Upper Receivers. Please try again later. Is the Subject Area "Wheat" applicable to this article?. The assemblies resulted in hundreds of thousands of sequence contigs per chromosome arm. Q-clones creating non-linear conformations were then fragmented and successive adaptive clustering algorithms were used to produce large contigs.
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Materials and Software Sharing. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No Is the Subject Area "Chromosome mapping" applicable to this article?. These sequences allowed the quantitative study of the chromosome arm sequence composition. Sum of PLOS and PubMed Central page views and downloads. We searched for cases where we find a homolog of a Brachypodium gene and a homolog of the UniGenes gene sequence next to it. Alternatively, it is possible that the UniGene sequence was derived from untranslated regions of the gene transcript.